My lab focuses on 3D structural studies of biological complexes using cryo-electron microscopy (cryoEM) and cryo-electron tomography (cryoET). These emerging methods are particularly suitable for structure determination of large molecular complexes, viruses, cellular machineries and bacterial cells. Recent efforts have focuses on developing and applying advanced cryoEM and cryoET techniques to visualize the dynamic processes of microbial infections and to decipher the mechanisms of fundamental biological processes. Our group is at the forefront in pushing the envelope of cryoEM reconstruction to atomic resolution. Research in my laboratory aims to understand the mechanisms governing macromolecular functions by pushing the resolution limit of cryoEM to near-atomic resolution and by describing large, pleiomorphic, dynamic structures or conformations using the integrative approach of cryoEM and cryoET. The long-term goal of our research is to study their structures and mechanisms of actions by an integrative approach using cryoEM/cryoET, bioinformatics modeling and high performance computing. Current efforts focus on three intermediate projects toward our goal: 1) to develop integrative techniques for atomic resolution cryoEM structural determination 2) to decipher the mechanisms of actions of multi-enzyme complexes and multi-component nanomachines, such as human pyruvate dehydrogenase complexes 3) to establish a 3D atlas of key events leading to the assembly and host infection of tumor herpesviruses.