Jimena Giudice, Ph.D.


Jimena Giudice, Ph.D.
Jimena Giudice
Assistant Professor
Cell Biology & Physiology
University of North Carolina at Chapel Hill
6340B Medical Biomolecular Research Building
111 Mason Farm Rd
City, State, ZIP
Chapel Hill, NC 27599
United States
(919) 962-6260
[email protected]
Research field
Molecular Biology; Developmental Biology
Award year
Country of origin
Mentor name
Dr. Thomas A. Cooper


Alternative splicing (AS) explains how a relatively limited number of genes gives rise to a vast proteomic complexity in higher eukaryotes. It was demonstrated that AS plays a key role during differentiation and development. During postnatal heart development many physiological changes are associated with dynamic regulation of gene expression. The fetal heart adapts to birth and converts to adult function through transcriptional and posttranscriptional mechanisms, including coordinated AS regulatory networks. The human heart is composed of cardiomyocytes (CM) (<20%), cardiac="" fibroblasts="" (cf)="" (~66%)="" and="" surface="" epithelium="" and="" vascular="" cells.="" communication="" between="" cf="" and="" cm="" should="" be="" present="" early="" in="" heart="" development,="" but="" few="" investigations="" have="" addressed="" this="" issue="" in="" vivo.="" we="" are="" studying="" splicing="" regulation="" during="" heart="" development="" specifically="" in="" cm="" and="" cf.="" our="" focus="" is="" on="" events="" directly="" regulated="" by="" rna="" binding="" proteins,="" which="" coordinate="" splicing="" networks,="" through="" a="" 10-fold="" down="" regulation="" within="" the="" first="" two="" weeks="" after="" birth.="" the="" network="" will="" be="" identified="" using="" genome="" wide="" analyses="" of="" rna-seq="" to="" identify="" as="" transitions="" and="" uv-crosslinking="" and="" immunoprecipitation="" to="" identify="" celf="" target="" rnas="" in="" vivo.="" rna-seq="" will="" be="" used="" to="" identify="" splicing="" transitions="" separately="" in="" cm="" and="" cf="" during="" postnatal="" mouse="" heart="" development.="" our="" goal="" is="" to="" determine="" how="" alternative="" splicing="" is="" initiated="" and="" controlled="" in="" the="" early="" development="" of="" the="" heart="" —="" work="" that="" will="" lead="" to="" an="" improved="" understanding="" of="" cardiac="">

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